Characterization of a dam mutant of Haemophilus influenzae Rd.

@article{Zaleski2004CharacterizationOA,
  title={Characterization of a dam mutant of Haemophilus influenzae Rd.},
  author={Piotr Zaleski and Andrzej Piekarowicz},
  journal={Microbiology},
  year={2004},
  volume={150 Pt 11},
  pages={3773-81}
}
The gene encoding Dam methyltransferase of Haemophilus influenzae was mutagenized by the insertion of a chloramphenicol-resistance cassette into the middle of the Dam coding sequence. This mutant construct was introduced into the H. influenzae chromosome by transformation and selection for Cam(R) transformants. The authors have shown that several phenotypic properties, resistance to antibiotics, dyes and detergent as well as efficiency of transformation, depend on the Dam methylation state of… CONTINUE READING