Characterization of a crucifer plant pre-rRNA processing complex.

Abstract

In cruciferous plants, the primary pre-rRNA cleavage site (P site) is immediately downstream of four similar, highly conserved sequences (A(1), A(2), A(3) and B) located within the 5'-ETS (5'-external transcribed spacer). In the present study, we describe the characterization of a plant NF D (nuclear factor D) that binds and interacts specifically with this A(123)BP cluster in the rDNA sequence. NF D is a high-molecular-mass complex containing nucleolin, fibrillarin and U3 and U14 snoRNAs. Furthermore, we show that NF D binds and cleaves pre-rRNA specifically at the P site. Thus we conclude that NF D is a pre-rRNA processing complex that may first assemble on rDNA and then bind nascent pre-rRNA.

Cite this paper

@article{SezVsquez2004CharacterizationOA, title={Characterization of a crucifer plant pre-rRNA processing complex.}, author={Julio S{\'a}ez-V{\'a}squez and D Caparros-Ruiz and Fr{\'e}dy Barneche and Manuel Echeverria}, journal={Biochemical Society transactions}, year={2004}, volume={32 Pt 4}, pages={578-80} }