Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins.

@article{Batten2006CharacterizationOA,
  title={Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins.},
  author={Carrie Anne Batten and Ian N Clarke and Sarah L. Kempster and Stefan L. Oliver and Janice C. Bridger and Paul R. Lambden},
  journal={Virology},
  year={2006},
  volume={356 1-2},
  pages={179-87}
}
The Southampton norovirus (SV) capsid protein was expressed as VLPs by recombinant baculoviruses in insect cells and was used to immunize mice for the production of monoclonal antibodies (mAbs). One mAb, CM54, showed broad cross-reactivity to genogroup I (GI) noroviruses, but was not reactive to GII capsid proteins. Interestingly mAb CM54 reacted to a bovine norovirus capsid protein. Immunoblot analysis indicated the binding site for CM54 was located in the shell domain between amino acid… CONTINUE READING