Characterization of a cell mutant specifically defective in the synthesis of anticoagulantly active heparan sulfate.

@article{ColliecJouault1994CharacterizationOA,
  title={Characterization of a cell mutant specifically defective in the synthesis of anticoagulantly active heparan sulfate.},
  author={S Colliec-Jouault and Nicholas W. Shworak and Jian Liu and Ariane I de Agostini and Robert Rosenberg},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 40},
  pages={24953-8}
}
We have investigated the characteristics of clonal L cell mutant VI-7 that has previously been demonstrated to exhibit reduced levels of cell surface proteoglycans containing heparan sulfate (HS) with regions of defined monosaccharide sequence that interact with antithrombin (HSact) (de Agostini, A. L., Lau, H. K., Leone, C., Youssoufian, H., and Rosenberg, R. D. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9784-9788). Pulse labeling revealed that the synthesis of HSact in mutant cells, as compared… CONTINUE READING

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