Characterization of a calcium-soluble protein fraction from yellow mustard (Sinapis alba) seed meal with potential application as an additive to calcium-rich drinks.

@article{Aluko2004CharacterizationOA,
  title={Characterization of a calcium-soluble protein fraction from yellow mustard (Sinapis alba) seed meal with potential application as an additive to calcium-rich drinks.},
  author={Rotimi Emmanuel Aluko and M. Foster Reaney and Tara McIntosh and François Ouellet and Felicitas M. Katepa-Mupondwa},
  journal={Journal of agricultural and food chemistry},
  year={2004},
  volume={52 19},
  pages={6030-4}
}
A calcium-soluble protein isolate (CSPI) was prepared from the supernatant obtained after addition of 0.75 M calcium chloride to a pH 5.0 aqueous extract of yellow mustard (Sinapis alba) seed meal. Total amino acid analysis showed that the CSPI has significantly higher (p < 0.05) contents of glutamic acid + glutamine, cysteine, and proline when compared to the precipitated, calcium-insoluble proteins. Peptide mass fingerprinting of tryptic peptides of the major polypeptides by mass spectrometry… CONTINUE READING