Characterization of a cDNA encoding cottonseed catalase.

  title={Characterization of a cDNA encoding cottonseed catalase.},
  author={Wenhua Ni and Rickie B. Turley and Richard N. Trelease},
  journal={Biochimica et biophysica acta},
  volume={1049 2},
A 1.7 kb cDNA clone was isolated from our lambda gt11 library constructed from poly(A) RNA of 24-h-old cotyledons. The cDNA encodes a full-length catalase peptide (492 amino acid residues). The calculated molecular mass is 56,800, similar to that determined for purified enzyme (57,000 SDS-PAGE). Among higher plant catalases, this cotton catalase shows the highest amino acid sequence identity (85%) to the subunit of homotetrameric maize CAT 1, a developmental counterpart to the homotetrameric… CONTINUE READING
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