Characterization of a bean (Phaseolus vulgaris L.) malic-enzyme gene.

  title={Characterization of a bean (Phaseolus vulgaris L.) malic-enzyme gene.},
  author={Michael Walter and Jacqueline Grima-Pettenati and Christian Feuillet},
  journal={European journal of biochemistry},
  volume={224 3},
We have isolated a genomic clone encoding a plant NADP(+)-dependent malic enzyme (NADP-ME). This clone, isolated from bean (Phaseolus vulgaris L.), covers the entire gene (exons, introns) and 5'-flanking regions. DNA sequencing defines 20 exons spanning approximately 4.5 kb, which range over 48-235 bp in size. All 19 introns are fairly small (79-391). The first intron resides in the 5'-untranslated leader sequence. Introns 10, 11 and 16 are located at positions identical to a rat malic-enzyme… CONTINUE READING