Characterization of a UV endonuclease gene from the fission yeast Schizosaccharomyces pombe and its bacterial homolog.

@article{Takao1996CharacterizationOA,
  title={Characterization of a UV endonuclease gene from the fission yeast Schizosaccharomyces pombe and its bacterial homolog.},
  author={Masashi Takao and Rie Yonemasu and Kazuo Yamamoto and Akira Yasui},
  journal={Nucleic acids research},
  year={1996},
  volume={24 7},
  pages={1267-71}
}
From the fission yeast Schizosaccharomyces pombe, a cDNA fragment was isolated, which confers UV resistance on repair deficient Escherichia coli host cells. The cloned cDNA encodes a protein of 68,815 Da, which has a 36.6% identity of amino acid sequence with the previously identified 74 kDa UV endonuclease of the filamentous fungus Neurospora crassa. Analysis of several truncated gene constructs shows that only the C-terminal two thirds region, which has 54% identity of amino acid sequence… CONTINUE READING