Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase.

  title={Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase.},
  author={Megan P. Hall and C Kiong Ho},
  volume={12 3},
The m7GpppN cap structure of eukaryotic mRNA is formed by the sequential action of RNA triphosphatase, guanylyltransferase, and (guanine N-7) methyltransferase. In trypanosomatid protozoa, the m7GpppN is further modified by seven methylation steps within the first four transcribed nucleosides to form the cap 4 structure. The RNA triphosphatase and guanylyltransferase components have been characterized in Trypanosoma brucei. Here we describe the identification and characterization of a T. brucei… CONTINUE READING