Characterization of a T-DNA promoter trap line of Arabidopsis thaliana uncovers a cryptic bi-directional promoter.

@article{Pratibha2013CharacterizationOA,
  title={Characterization of a T-DNA promoter trap line of Arabidopsis thaliana uncovers a cryptic bi-directional promoter.},
  author={Pritu Pratibha and Sunil Kumar Singh and Isha Sharma and Rajendra Kumar and Ramamurthy Srinivasan and Shripad Ramachandra Bhat and Paramvir Singh Ahuja and Yelam Sreenivasulu},
  journal={Gene},
  year={2013},
  volume={524 1},
  pages={
          22-7
        }
}
Investigation of the transgenic Arabidopsis promoter trap line GFP-868 that showed GFP expression only in anthers revealed the T-DNA insertion at 461bp upstream to the hypothetical gene At4g10596 with the GFP reporter gene in head-to-head orientation to the At4g10596 gene. The expression of the At4g10596 gene in wild type and in GFP-868 plant homozygous for T-DNA insertion was comparable and found in all tissues tested, while the GFP expression was restricted to anthers of the GFP-868 plants… CONTINUE READING
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