Characterization of a PSE-4 mutant with different properties in relation to penicillanic acid sulfones: importance of residues 216 to 218 in class A beta-lactamases.

@article{Sabbagh1998CharacterizationOA,
  title={Characterization of a PSE-4 mutant with different properties in relation to penicillanic acid sulfones: importance of residues 216 to 218 in class A beta-lactamases.},
  author={Yves Sabbagh and Elizabeth C Theriault and François Sanschagrin and Normand Voyer and Timothy G. Palzkill and Roger C. L{\'e}vesque},
  journal={Antimicrobial agents and chemotherapy},
  year={1998},
  volume={42 9},
  pages={2319-25}
}
Class A beta-lactamases are inactivated by the suicide inactivators sulbactam, clavulanic acid, and tazobactam. An examination of multiple alignments indicated that amino acids 216 to 218 differed among class A enzymes. By random replacement mutagenesis of codons 216 to 218 in PSE-4, a complete library consisting of 40,864 mutants was created. The library of mutants with mutations at positions 216 to 218 in PSE-4 was screened on carbenicillin and ampicillin with the inactivator sulbactam; a… CONTINUE READING