Characterization of a NO(3)-Sensitive H-ATPase from Corn Roots.

  title={Characterization of a NO(3)-Sensitive H-ATPase from Corn Roots.},
  author={S D O'neill and Alan B Bennett and Roger M. Spanswick},
  journal={Plant physiology},
  volume={72 3},
When assayed in the presence of azide, NO(3) (-) was shown to be a specific inhibitor of a proton-translocating ATPase present in corn (Zea mays L. cv WF9 x M017) root microsomal membranes. The distribution of the NO(3) (-)-sensitive ATPase on sucrose gradients and its general characteristics are similar to those previously reported for the anion-stimulated H(+)-ATPase of corn roots believed to be of tonoplast origin. An ATPase inhibited by 20 mum vanadate and insensitive to molybdate was also… CONTINUE READING
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