Characterization of a Madin-Darby canine kidney cell line stably expressing TRPV5

  title={Characterization of a Madin-Darby canine kidney cell line stably expressing TRPV5},
  author={Els den Dekker and Joost P. H. Schoeber and Catalin N. Topala and Stan F. J. van de Graaf and Joost G. J. Hoenderop and Ren{\'e} J M Bindels},
  journal={Pfl{\"u}gers Archiv},
To provide a cell model for studying specifically the regulation of Ca2+ entry by the epithelial calcium channel transient receptor potential-vanilloid-5 (TRPV5), green fluorescent protein (GFP)-tagged TRPV5 was expressed stably in Madin-Darby canine kidney type I (MDCK) cells. The localization of GFP-TRPV5 in this cell line showed an intracellular granular distribution. Ca2+ uptake in GFP-TRPV5-MDCK cells cultured on plastic supports was threefold higher than in non-transfected cells. Moreover… CONTINUE READING


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