Characterization of a Generalized Transducing Phage of Poly-(gamma)-Glutamic Acid-Producing Bacillus subtilis and Its Application for Analysis of Tn917-LTV1 Insertional Mutants Defective in Poly-(gamma)-Glutamic Acid Production.

@article{Nagai1997CharacterizationOA,
  title={Characterization of a Generalized Transducing Phage of Poly-(gamma)-Glutamic Acid-Producing Bacillus subtilis and Its Application for Analysis of Tn917-LTV1 Insertional Mutants Defective in Poly-(gamma)-Glutamic Acid Production.},
  author={Takashi Nagai and Yuichiro Itoh},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 10},
  pages={
          4087-9
        }
}
A generalized transducing phage, (phi)BN100, was isolated from a Bacillus subtilis (natto) strain producing poly-(gamma)-glutamic acid ((gamma)PGA). Transduction frequencies for a given marker ranged from 3.8 x 10(sup-8) to 1.6 x 10(sup-6) per phage particle. The genome size of the phage was approximately 42 kb. (phi)BN100 was used successfully to identify bona fide Tn917-LTV1 transpositional mutants defective in (gamma)PGA production. 

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SHOWING 1-10 OF 16 REFERENCES

Plasmid vectors for recovering and exploiting Tn917 transpositions in Bacillus and other gram-positive bacteria, p. 79–103

P. Youngman
  • IRL Press, Oxford, England. VOL
  • 1989
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

Bacillus anthracis, p. 113–124

C. B. Thorne
  • 1993
VIEW 1 EXCERPT

Genetic exchange and homologous recombination , p . 555 – 584

J. A. Hock, R. Losic
  • Bacillus subtilis and other gram - positive bacteria : biochemistry , physiology , and molecular genet
  • 1993

Industrial application of B . subtilis , p . 143 – 161 . In B . Maruo and H . Yoshikawa ( ed . ) , Bacillus subtilis : molecular biology and industrial application

S. Ueda
  • 1989

Industrial application of B. subtilis, p. 143–161

S. Ueda
  • Kodansha Ltd.,
  • 1989
VIEW 1 EXCERPT