Characterization of a Deep-Sea Sediment Metagenomic Clone that Produces Water-Soluble Melanin in Escherichia coli

  title={Characterization of a Deep-Sea Sediment Metagenomic Clone that Produces Water-Soluble Melanin in Escherichia coli},
  author={Yali Huang and X.-T. Lai and Xiaocui He and L. Cao and Z. Zeng and Jiong Zhang and Shining Zhou},
  journal={Marine Biotechnology},
To access to the microbial genetic resources of deep-sea sediment by a culture-independent approach, the sediment DNA was extracted and cloned into fosmid vector (pCC1FOS) generating a library of 39,600 clones with inserts of 24–45 kb. The clone fss6 producing red-brown pigment was isolated and characterized. The pigment was identified as melanin according to its physico-chemical characteristics. Subcloning and sequences analyses of fss6 demonstrated that one open reading frame (ORF2) was… Expand
Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library
Mining a South African deep mine metagenome for the discovery of novel biocatalysts
Seasonal and spatial diversity of microbial communities in marine sediments of the South China Sea


Characterization of a Forest Soil Metagenome Clone That Confers Indirubin and Indigo Production on Escherichia coli
Isolation of Antibiotics Turbomycin A and B from a Metagenomic Library of Soil Microbial DNA
Genome sequence of the deep-sea γ-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy
  • S. Hou, Jimmy H. Saw, +19 authors M. Alam
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea.
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea
Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov.
Idiomarina loihiensis sp. nov., a halophilic gamma-Proteobacterium from the Lō'ihi submarine volcano, Hawai'i.
Expression and isolation of antimicrobial small molecules from soil DNA libraries.