Characterization of a Chlamydomonas reinhardtii mutant strain with improved biomass production under low light and mixotrophic conditions

@article{Zhou2015CharacterizationOA,
  title={Characterization of a Chlamydomonas reinhardtii mutant strain with improved biomass production under low light and mixotrophic conditions},
  author={Yan Zhou and Lance Schideman and D. S. Park and Alexandrina D. Stirbet and Govindjee and S. Indu Rupassara and J. D. Krehbiel and Manfredo J. Seufferheld},
  journal={Algal Research-Biomass Biofuels and Bioproducts},
  year={2015},
  volume={11},
  pages={134-147}
}
Key Methodarticle i nfo Chlamydomonas reinhardtii Algal biofuels Chlorophyll fluorescence transient Metabolite profiling Knock-out mutant Non-photochemical quenching Mixotrophic condition Biophysical and biochemical characteristics of a spontaneous "mutant" strain (IM) of Chlamydomonas reinhardtii were quantified and compared with its progenitor (KO), a "knock-out" mutant with defects in phototaxis, and to its wild-type (WT); defects were shown to be preserved in the IM mutant. Growth curves showed that…Expand

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References

SHOWING 1-10 OF 148 REFERENCES
Mutants of Chlamydomonas reinhardtii resistant to very high light
TLDR
The ability of these mutants to survive under VHL in 5% CO 2 evidently involves processes other than photoprotection via the xanthophyll cycle and resistance to photodamage via alterations in D1 function.
Improving the Photosynthetic Productivity and Light Utilization in Algal Biofuel Systems : Metabolic and Physiological Characterization of a Potentially Advantageous Mutant of Chlamydomonas Reinhardtii
In this study, we report initial biophysical and biochemical characterization of a spontaneous ‘mutant’ (referred to as ‘IM’) of the green alga Chlamydomonas reinhardtii with several unique
A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain
Abstract Photoproduction of H2 gas was examined in the Chlamydomonas reinhardtii tla1 strain, CC-4169, containing a truncated light-harvesting antenna, along with its parental CC-425 strain. Although
Effects of acetate on facultative autotrophy in Chlamydomonas reinhardtii assessed by photosynthetic measurements and stable isotope analyses.
TLDR
It is found that mixotrophic growth in acetate is not associated with activation of the cyanide-insensitive alternative oxidase pathway, and interpretations of the effects of environmental or genetic manipulations of photosynthesis are likely to be confounded by acetate in the medium.
The Occurrence of the psbS Gene Product in Chlamydomonas reinhardtii and in Other Photosynthetic Organisms and Its Correlation with Energy Quenching †
TLDR
By correlating growth habitat and PsbS accumulation in different species, it is suggested that during the evolution the light environment has been a determinant factor for the conservation/loss of the psbS function.
tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size
TLDR
The tla1 strain required a higher light intensity for the saturation of photosynthesis and showed greater solar conversion efficiencies and a higher photosynthetic productivity than the wild type under mass culture conditions.
Acclimation of Chlamydomonas reinhardtii to Different Growth Irradiances*
TLDR
Higher plants and green algae have evolved different acclimatizing mechanisms, and cells acclimated to low light intensity are indeed able to produce more biomass per photon available as compared with high light-acclimated cells, which dissipate as heat a large part of light absorbed, thus reducing their photosynthetic efficiency.
Hydrogen Photo-Production by Mixotrophic Cultivation of Chlamydomonas Reinhardtii: Interaction between Organic Carbon and Nitrogen
Hydrogen photo-production by a wild type and two engineered strains of Chlamydomonas reinhardtii was investigated. Growth rate values and hydrogen yields attained as the concentration of acetate and
Acclimation of Chlamydomonas reinhardtii to its nutrient environment.
TLDR
This article will focus on the acclimation of Chlamydomonas reinhardtii, a unicellular, eukaryotic green alga to conditions of nitrogen, sulfur and phosphorus deprivation and establishing relationships between the responses of this morphologically simple, photosynthetic eUKaryote and those of both nonphotosynthetic organisms and vascular plants.
Chlamydomonas reinhardtii as the photosynthetic yeast.
  • J. Rochaix
  • Biology, Medicine
    Annual review of genetics
  • 1995
TLDR
The green unicellular alga Chlamydomonas reinhardtii has long been used as a model system for studying photosynthesis, chloroplast biogenesis, and flagellar function and assembly, and it offers additional new promising areas of research, such aschloroplast-mitochondrial interactions, phototransduction, and the behavioral response to light.
...
1
2
3
4
5
...