Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum.

@article{Nlling1995CharacterizationOA,
  title={Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum.},
  author={J{\"o}rk N{\"o}lling and Miho Ishii and J{\"u}rgen Koch and T D Pihl and John N Reeve and Rudolf Kurt Thauer and Reiner Hedderich},
  journal={European journal of biochemistry},
  year={1995},
  volume={231 3},
  pages={628-38}
}
Methanobacterium thermoautotrophicum strains contain a flavoprotein (flavoprotein A) that copurifies with the H2:heterodisulfide oxidoreductase complex. In this study, we report the iron-dependent synthesis and biochemical properties of flavoprotein A, cloning and sequencing of the flavoprotein-A-encoding gene (fpaA) and the co-transcription of fpaA with two downstream open reading frames, one of which (rdxA) appears to encode a rubredoxin. Native flavoprotein A has been shown to be a homodimer… CONTINUE READING