Characterization of Unique Small RNA Populations from Rice Grain

  title={Characterization of Unique Small RNA Populations from Rice Grain},
  author={Sara E. Heisel and Yuanji Zhang and Edwards Allen and Liang Guo and Tracey L. Reynolds and Xiao Yang and David Kovalic and James K. Roberts},
  journal={PLoS ONE},
  pages={e219 - 90}
Small RNAs (approximately 20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways in plants and animals. Here we analyze small RNA populations from mature rice grain and seedlings by pyrosequencing. Using a clustering algorithm to locate regions producing small RNAs, we classified hotspots of small RNA generation within the genome. Hotspots here are defined as 1 kb regions within which small RNAs are significantly overproduced relative to the rest of… CONTINUE READING
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