Characterization of Two Novel Neuropeptides From the Sea Cucumber Holothuria glaberrima.

@article{DazMiranda1992CharacterizationOT,
  title={Characterization of Two Novel Neuropeptides From the Sea Cucumber Holothuria glaberrima.},
  author={L. D{\'i}az-Miranda and D. Price and M. Greenberg and T. Lee and K. Doble and J. Garc{\'i}a-Arrar{\'a}s},
  journal={The Biological bulletin},
  year={1992},
  volume={182 2},
  pages={
          241-247
        }
}
Two peptides were purified from intestinal extracts of a sea cucumber, Holothuria glaberrima, by high pressure liquid chromatography (HPLC). The peptides were detected by a radioimmunoassay (RIA) based on an antiserum raised to the molluscan peptide, pGlu-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (pQDPFLRFamide). Automated sequencing and mass spectrometry indicate that the isolated peptides are: Gly-Phe-Ser-Lys-Leu-Tyr-Phe-NH2 (GFSKLYFamide) and Ser-Gly-Tyr-Ser-Val-Leu-Tyr-Phe-NH2 (SGYSVLYFamide). These are… Expand
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Pharmacological action of the heptapeptide GFSKLYFamide in the muscle of the sea cucumber Holothuria glaberrima (Echinodermata).
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