Characterization of Structure and Cellular Components of Aspirated and Excised Adipose Tissue

  title={Characterization of Structure and Cellular Components of Aspirated and Excised Adipose Tissue},
  author={Hitomi Eto and Hirotaka Suga and Daisuke Matsumoto and Keita Inoue and Noriyuki Aoi and Harunosuke Kato and Jun Araki and Kotaro Yoshimura},
  journal={Plastic and Reconstructive Surgery},
Background: Adipose tissue is an easily accessible tissue for use as a soft-tissue filler and source of adult multipotent cells, called adipose-derived stem/stromal/progenitor cells. However, many aspects of its anatomy remain unclear because of the fragile structure of adipocytes and adipose tissue. Methods: Aspirated (n = 15) or intact (n = 9) subcutaneous adipose tissue samples were evaluated by (1) whole-mount histology with triple-fluorescence staining for three-dimensional visualization… 

Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering

It is shown that adipose tissue provides for an excellent source of endothelial cells for tissue engineering purposes, since they are readily available, and easily isolated and amplified.

Adipose Stem Cells: From Liposuction to Adipose Tissue Engineering

A large volume of adipose tissue can be easily harvested through a well-established cosmetic surgical procedure, liposuction. Mesenchymal adherent stem/progenitor cells, similar to those from bone

The Fate of Adipocytes after Nonvascularized Fat Grafting: Evidence of Early Death and Replacement of Adipocytes

Significant evidence is shown of very dynamic remodeling of adipose tissue after nonvascularized grafting and of repair/regeneration of the dead tissue had begun.

Measured Levels of Human Adipose Tissue-Derived Stem Cells in Adipose Tissue Is Strongly Dependent on Harvesting Method and Stem Cell Isolation Technique.

The study indicates that harvesting by aspiration halves the concentration of adipose tissue-derived stem cells in adipOSE tissue samples when compared to structural intact adipose tissues, presenting stem cell yield higher than previously described in the current literature.

In vivo manipulation of stem cells for adipose tissue repair/reconstruction.

Careful design of the microenvironment, cell delivery protocol to avoid unexpected behavior and induce maximal potential, and selection of target diseases, will be critical to the success of clinical applications of adipose-derived stromal cells.

Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine

A simple and efficient method to isolate adipose-derived mesenchymal stem cells from different preparations, and the cells isolated from the liquid fat tissue grow faster at higher passages, and significantly more cells display MSC markers.

The Survival Condition and Immunoregulatory Function of Adipose Stromal Vascular Fraction (SVF) in the Early Stage of Nonvascularized Adipose Transplantation

The study suggests that the survival of adipose tissue after nonvascularized adipose transplantation may be due to the ASCs in SVF cells, and the immunoregulatory function of SVF Cells may be indirectly contributing to the remolding of adiposes transplant, which may lead to SVF-enriched adipose transplants.

Adipose tissue: A valuable resource of biomaterials for soft tissue engineering

Extracellular matrices, isolated through decellularization of mammalian tissues, have been successfully used in a variety of tissue engineering and regenerative medicine applications and their use in soft tissue engineering is reviewed.

Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration

It is concluded that these two methods of obtaining adipose tissue do not systematically differ in the results of cytokine mRNA content, cellular senescence or stromovascular cell composition.

Fractionation of Adipose Tissue Procedure With a Disposable One-Hole Fractionator.

The FAT procedure with a disposable one-hole fractionator effectively isolates tSVF with a comparable cell count and cell composition in comparison with the three-hole reusable fractionator.



Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection.

Comparisons of histologic features and yield of adipose-derived stromal (stem) cells (ASCs) were compared between human aspirated fat and excised whole fat to suggest clinical potential of the CAL method for soft tissue augmentation and partly explain why transplanted aspiratedFat does not survive well.

Numerical Measurement of Viable and Nonviable Adipocytes and Other Cellular Components in Aspirated Fat Tissue

Single use or a combination of the viability assays used in this study can appropriately determine the number of adipocytes and other cells, although it remains difficult to assess original cells directly without tissue dissociation.

Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

The results show clearly that the yield of preadipocytes isolated from liposuction material (within 60 min after extraction and after 24 h of storage) is higher than the cell yield from excised adipose tissue, which proves that extraction by suction does not damage the stromal cell fraction in the adiposes tissue.

Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation.

A novel transplantation strategy, termed cell-assisted lipotransfer, involves the enrichment of adipose progenitor cells in grafts; preliminary results suggest this approach to be safe and effective.

Human adipose tissue is a source of multipotent stem cells.

To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.

Defining stem and progenitor cells within adipose tissue.

It is proposed that adipose tissue-derived stem cells (ADSC) are vascular precursor (stem) cells at various stages of differentiation in their native tissue, which can differentiate into tissue-specific cells such as adipocytes.

Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels

Living tissue imaging techniques provide novel evidence of the dynamic interactions between differentiating adipocytes, stromal cells, and angiogenesis in living obese adipose tissue.

Cellular characterization of adipose tissue from various body sites of women.

The findings suggest that the increase in aromatase expression with advancing age and the higher aromatases expression in the hip compared with the abdomen in women may be caused by alterations in specific signal transduction mechanisms rather than a simple increase in local adipose fibroblast numbers.

Identification of White Adipocyte Progenitor Cells In Vivo