Characterization of Staphylococcus aureus cell wall glycan strands, evidence for a new beta-N-acetylglucosaminidase activity.

@article{Boneca2000CharacterizationOS,
  title={Characterization of Staphylococcus aureus cell wall glycan strands, evidence for a new beta-N-acetylglucosaminidase activity.},
  author={Ivo G Boneca and Zhi Hua Huang and Douglas A. Gage and Alexander Tomasz},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 14},
  pages={9910-8}
}
Using sequential digestion with the glycyl-glycine endopeptidase lysostaphin followed by the pneumococcal N-acetylmuramyl-L-alanine amidase (amidase), the glycan strands of the peptidoglycan of Staphylococcus aureus were purified and analyzed by a combination of reverse-phase-high pressure liquid chromatography (HPLC) and mass spectrometry. Reverse-phase-HPLC resolved the glycan strands to a family of major peaks, which represented oligosaccharides composed of repeating disaccharide units (N… CONTINUE READING

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