Characterization of Schizosaccharomyces pombe malate permease by expression in Saccharomyces cerevisiae.

  title={Characterization of Schizosaccharomyces pombe malate permease by expression in Saccharomyces cerevisiae.},
  author={Carole Camarasa and François-Cl{\'e}ment Bidard and M Bony and Pierre Barr{\'e} and Sylvie Dequin},
  journal={Applied and environmental microbiology},
  volume={67 9},
In Saccharomyces cerevisiae, L-malic acid transport is not carrier mediated and is limited to slow, simple diffusion of the undissociated acid. Expression in S. cerevisiae of the MAE1 gene, encoding Schizosaccharomyces pombe malate permease, markedly increased L-malic acid uptake in this yeast. In this strain, at pH 3.5 (encountered in industrial processes), L-malic acid uptake involves Mae1p-mediated transport of the monoanionic form of the acid (apparent kinetic parameters: Vmax = 8.7 nmol/mg… CONTINUE READING
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Malolactic fermentation in grape musts by a genetically engineered strain of Saccharomyces cerevisiae

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