Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.

@article{Ella1996CharacterizationOS,
  title={Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.},
  author={Krishna Ella and Joseph W. Dolan and Chao Qi and K. Eberhard Meier},
  journal={The Biochemical journal},
  year={1996},
  volume={314 ( Pt 1)},
  pages={15-9}
}
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.