Characterization of SRp46, a Novel Human SR Splicing Factor Encoded by a PR264/SC35 Retropseudogene

@article{Soret1998CharacterizationOS,
  title={Characterization of SRp46, a Novel Human SR Splicing Factor Encoded by a PR264/SC35 Retropseudogene},
  author={Johann Soret and Renata Gattoni and C{\'e}cile Guyon and Alain Sureau and Michel Popielarz and Erwann le Rouzic and St{\'e}phanie Dumon and Françoise Apiou and Bernard Dutrillaux and Hartmut Voss and Wilhelm J. Ansorge and James St{\'e}venin and Bernard Perbal},
  journal={Molecular and Cellular Biology},
  year={1998},
  volume={18},
  pages={4924 - 4934}
}
ABSTRACT The highly conserved SR family contains a growing number of phosphoproteins acting as both essential and alternative splicing factors. In this study, we have cloned human genomic and cDNA sequences encoding a novel SR protein designated SRp46. Nucleotide sequence analyses have revealed that the SRp46 gene corresponds to an expressed PR264/SC35 retropseudogene. As a result of mutations and amplifications, the SRp46 protein significantly differs from the PR264/SC35 factor, mainly at the… 
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TLDR
It is found that RBD2 is essential for function and determines the specificity of RBD1 in Srp2, which is consistent with the notion that the RBDs of these proteins may be involved in pre-mRNA recognition.
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
TLDR
The results provide the first evidence for cooperation between an RBD and a zinc knuckle in defining the specificity of an RNA binding domain and demonstrate the ability of 9G8 and of its zincknuckle variant, or SRp20, to act as efficient splicing transactivators through their specific RNA targets.
Serine-Arginine (SR) Protein-like Factors That Antagonize Authentic SR Proteins and Regulate Alternative Splicing*
TLDR
It is proposed that these two RNA-binding proteins are SR protein-like alternative splicing regulators that antagonize authentic SR proteins in the modulation of alternative 5′ splice site choice.
Functional characterization of SR and SR‐related genes in Caenorhabditis elegans
The SR proteins constitute a family of nuclear phosphoproteins, which are required for constitutive splicing and also influence alternative splicing regulation. Initially, it was suggested that SR…
SC35 autoregulates its expression by promoting splicing events that destabilize its mRNAs
TLDR
It is shown that overexpression of SC35 in HeLa cells results in a significant decrease of endogenous SC35 mRNA levels along with changes in the relative abundance ofSC35 alternatively spliced mRNAs.
The Modular Domain Structure of ASF/SF2: Significance for its Function as a Regulator of RNA Splicing
TLDR
The results suggest that the RBD2 domain in ASF/SF2 plays the most decisive role in the alternative 5’ and 3’ splice site regulatory activities of ASF-RBD2.
Broad specificity of SR (serine/arginine) proteins in the regulation of alternative splicing of pre-messenger RNA.
Characterization of the mouse hnRNP A2/B1/B0 gene and identification of processed pseudogenes.
Systematic genome-wide annotation of spliceosomal proteins reveals differential gene family expansion.
TLDR
This work describes a semiautomated computational pipeline to identify and annotate splicing factors in representative species of eukaryotes and reports several intronless genes amongst splicing proteins in mammals, suggesting that retrotransposition contributed to the complexity of the mammalian splicing apparatus.
SRp30a (ASF/SF2) regulates the alternative splicing of caspase-9 pre-mRNA and is required for ceramide-responsiveness Published, JLR Papers in Press, February 27, 2006.
TLDR
The specific downregulation of SRp30a was identified as an RNA trans-acting factor that functions as a major regulator of caspase-9 pre-mRNA processing and is required for ceramide to mediate the alternative splicing of cospase- 9.
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References

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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