Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing

@article{Suzuki2006CharacterizationOR,
  title={Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing},
  author={Hitoshi Suzuki and Yuhong Zuo and Jinhua Wang and Michael Q Zhang and Arun Malhotra and Akila Mayeda},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={e63 - e63}
}
Besides linear RNAs, pre-mRNA splicing generates three forms of RNAs: lariat introns, Y-structure introns from trans-splicing, and circular exons through exon skipping. To study the persistence of excised introns in total cellular RNA, we used three Escherichia coli 3' to 5' exoribonucleases. Ribonuclease R (RNase R) thoroughly degrades the abundant linear RNAs and the Y-structure RNA, while preserving the loop portion of a lariat RNA. Ribonuclease II (RNase II) and polynucleotide phosphorylase… CONTINUE READING

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