Characterization of Pathogenic Escherichia coli in River Water by Simultaneous Detection and Sequencing of 14 Virulence Genes.

@article{Gomi2015CharacterizationOP,
  title={Characterization of Pathogenic Escherichia coli in River Water by Simultaneous Detection and Sequencing of 14 Virulence Genes.},
  author={Ryota Gomi and Tomonari Matsuda and Yuji Fujimori and Hidenori Harada and Yasuto Matsui and Minoru Yoneda},
  journal={Environmental science \& technology},
  year={2015},
  volume={49 11},
  pages={
          6800-7
        }
}
The occurrence of pathogenic Escherichia coli in environmental waters increases the risk of waterborne disease. In this study, 14 virulence genes in 669 E. coli isolates (549 isolates from the Yamato River in Japan, and 30 isolates from each of the following hosts: humans, cows, pigs, and chickens) were simultaneously quantified by multiplex PCR and dual index sequencing to determine the prevalence of potentially pathogenic E. coli. Among the 549 environmental isolates, 64 (12%) were classified… 
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