Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome.

@article{Kim2015CharacterizationON,
  title={Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome.},
  author={Hee Jung Kim and Yu Seok Jeong and Won Kyeong Jung and Sung Kyum Kim and Hyun Woo Lee and Hyung Yeel Kahng and Jungho Kim and Hoon Kim},
  journal={Molecular biotechnology},
  year={2015},
  volume={57 9},
  pages={
          781-92
        }
}
Two genes encoding lipolytic enzymes were isolated from a metagenomic library constructed from oil-polluted mud flats. An esterase gene, est3K, encoded a protein of 299 amino acids (ca. 32,364 Da). Est3K was a family IV esterase with typical motifs, HGGG, and HGF. Although est3K showed high identity to many genes with no information on their enzymatic properties, Est3K showed the highest identity (36 %) to SBLip5.1 from forest soil metagenome when compared to the enzymes with reported… CONTINUE READING
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