Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution
@article{Marais2015CharacterizationON, title={Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution}, author={Armelle Marais and Chantal Faure and Eldar Mustafayev and Thierry Candresse}, journal={PLoS ONE}, year={2015}, volume={10} }
Double stranded RNAs from Prunus samples gathered from various surveys were analyzed by a deep-sequencing approach. Contig annotations revealed the presence of a potential new viral species in an Azerbaijani almond tree (Prunus amygdalus) and its genome sequence was completed. Its genomic organization is similar to that of the recently described Apricot vein clearing associated virus (AVCaV) for which two new isolates were also characterized, in a similar fashion, from two Japanese plums…
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Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)
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Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed.
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Critical genome information of Asian prunus virus 1, APV2 and APV3 is provided, showing that the unusually long 3’ non-coding region (3' NCR) is highly variable and a hot spot for indel polymorphisms.
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- Biology
- 2017
Next generation sequencing (NGS) of total RNAs from a wild cherry tree revealed a multiple virus infection and evidence was obtained supporting the recombinant nature of two Slovak isolates and for the presence of “non-cherry” group isolates of CVA in several cherry host plants.
Partial biological and molecular characterization of a novel citrivirus from Nandina domestica
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Computer-based analysis suggests that this novel virus isolated from Nandina domestica ‘Firepower’ in Auckland, New Zealand is the result of recombination between CLBV-Act and an unknown virus, highlighting the importance of this phenomenon for betaflexivirus evolution.
Small RNA NGS Revealed the Presence of Cherry Virus A and Little Cherry Virus 1 on Apricots in Hungary
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- 2018
C Cherry virus A (CVA) and little cherry virus 1 (LChV-1) are identified for the first time in Hungary using next generation sequencing of small RNAs to investigate their phylogenetic relationships.
Identification and characterization of a novel rhabdovirus infecting peach in China.
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Virus classification based on in-depth sequence analyses and development of demarcation criteria using the Betaflexiviridae as a case study.
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Current issues concerning classification of viruses in the family Betaflexiviridae in the era of high-throughput sequencing are critically addressed and an updated set of demarcation criteria based on a process involving pairwise identity analyses and phylogenetics are proposed.
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