Characterization of NADP-isocitrate dehydrogenase expression in a carrot mutant cell line with enhanced citrate excretion

@article{Kihara2004CharacterizationON,
  title={Characterization of NADP-isocitrate dehydrogenase expression in a carrot mutant cell line with enhanced citrate excretion},
  author={Tomonori Kihara and Takashi Ohno and Hiroyuki Koyama and Takuya Sawafuji and Tetsuo Hara},
  journal={Plant and Soil},
  year={2004},
  volume={248},
  pages={145-153}
}
Citrate in the cytosol is converted to isocitrate, by the action of aconitase, and then isocitrate is converted to 2-oxoglutarate by the action of NADP-specific isocitrate dehydrogenase (NADP-ICDH). This pathway is modified in a mutant carrot (Daucus carotaL.) cell line [designated as insoluble phosphate grower (IPG): Koyama et al., 1992 Plant Cell Physiol. 33, 173–177], which releases large amounts of the citrate and grows faster than the wild-type cells in Al-phosphate medium. In the current… CONTINUE READING