Characterization of Mycobacterium smegmatis mutants defective in 1-d-myo-inosityl-2-amino-2-deoxy-alpha-d-glucopyranoside and mycothiol biosynthesis.

@article{Newton1999CharacterizationOM,
  title={Characterization of Mycobacterium smegmatis mutants defective in 1-d-myo-inosityl-2-amino-2-deoxy-alpha-d-glucopyranoside and mycothiol biosynthesis.},
  author={Gerald L. Newton and Mia D. Unson and Stefan Anderberg and Joe A. Aguilera and Nuri Oh and Stephen Delcardayre and Yossef Av-Gay and Robert Fahey},
  journal={Biochemical and biophysical research communications},
  year={1999},
  volume={255 2},
  pages={
          239-44
        }
}
Mycothiol (MSH) is the major low molecular weight thiol in mycobacteria. Two chemical mutants with low MSH and one with no MSH (strain 49) were produced in Mycobacterium smegmatis mc2155 to assess the role of MSH in mycobacteria. Strain 49 was shown to not produce 1-d-myo-inosityl-2-amino-2-deoxy-alpha-d-glucopyranoside (GlcN-Ins), an intermediate in MSH biosynthesis. Relative to the parent strain, mutant 49 formed colonies more slowly on solid media and was more sensitive to H2O2 and rifampin… CONTINUE READING

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