Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex

@article{Lorenzen1993CharacterizationOM,
  title={Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex},
  author={Anna Lorenzen and Monika Nitsch‐Kirsch and Heidrun Vogt and Ulrich Schwabe},
  journal={Journal of Neurochemistry},
  year={1993},
  volume={60}
}
Abstract: A high‐affinity binding site for 5′‐N‐ethylcarboxamido[3H]adenosine ([3H]NECA) from bovine cerebral cortex has been characterized in its membrane‐bound and solubilized state after gel filtration on Sepharose CL‐6B. For detection of this site in membranes, it was necessary to remove metabolites with high affinities for this site enzymatically, e.g., adenosine by addition of adenosine deaminase and inosine by addition of nucleoside phosphorylase. The pore‐forming peptide antibiotic… 
View on Wiley
onlinelibrary.wiley.com
7 Citations
Background Citations
2

7 Citations

Citation Type

Citation Type

Characterization of a novel adenosine binding protein sensitive to cyclic AMP in rat brain cytosolic and particulate fractions.
Evidence for the Functional Expression and Pharmacological Characterization of Adenine Receptors in Native Cells and Tissues
TLDR
It is shown for the first time that the adenine receptor is expressed in membrane preparations of native tissues and cell lines in high density, including rat brain cortex, rat brain striatum, and the mouse neuroblastoma × rat glioma hybrid cell line NG108-15.
Tritium-labeled agonists as tools for studying adenosine A2B receptors
TLDR
Results indicate that nucleoside and non-nucleoside agonists are binding to very different conformations of the A2BAR.
Nomenclature and classification of purinoceptors.
TLDR
The evidence is now compelling that ATP plays important physiological and/ or pathophysiological roles in a variety of biological systems, and the presence of receptors for ADP and adenosine (presumably A2) receptors exist on platelets is compelling.
Adenosine receptors and their modulators.
VI. Nomenclature and Classification of Purinoceptors*
TLDR
The evidence that pumine nucleotides and nucleosides are natural ligands for a wide class of receptors is summarized.
B. Localization and Function of Pt Receptors

References

SHOWING 1-10 OF 36 REFERENCES
Purification and characterization of the adenosine A2-like binding site from human placental membrane.
Heterogeneity of binding sites for N-ethylcar☐amido[3H]adenosine in rat brain: Effects of N-ethylmaleimide
Characterization of the A2 adenosine receptor labeled by [3H]NECA in rat striatal membranes.
TLDR
The regional distribution of [3H]NECA binding and the affinities of adenosine agonists and antagonists for inhibition of binding indicate that the site labeled by [3NECA belongs to the high affinity, or A2a, subclass of A2 receptor.
Identification of the A2 adenosine receptor binding subunit by photoaffinity crosslinking.
TLDR
125I-labeled PAPA-APEC has identified the A2 receptor binding sub unit as a 45-kDa protein that is unique and distinct from the A1 binding subunit.
Autoradiographic evidence for multiple CNS binding sites for adenosine derivatives
Ligand binding to the adenine analogue binding protein of the rabbit erythrocyte.
TLDR
Inhibition of [3H]adenosine binding by a series of 77 adenosine analogues was used to define the factors determining the binding affinity of this nucleoside.
Ligand binding to the adenine analogue binding protein of the rabbit erythrocyte.
TLDR
Inhibition of [3H]adenosine binding by a series of 77 adenosine analogues was used to define the factors determining the binding affinity of this nucleoside.
Interaction of Adenosine with Adenosine-Binding Protein, S-Adenosylhomocysteine Hydrolase
TLDR
This chapter is a brief review of the properties of AdoHcy hydrolase, with emphasis on the interaction of the enzyme with Ado.
Modification of receptor-mediated inhibition of adenylate cyclase in NG108-15 neuroblastoma X glioma cells by n-ethylmaleimide.
TLDR
Using membranes derived from NG108-15 neuroblastoma X glioma cells as a model system, the effect of NEM is examined on the interaction of three inhibitory receptors with adenylate cyclase and a 30-fold decrease in the potency of epinephrine is observed.
Agonist-independent inhibition of G protein activation by muscarinic acetylcholine receptor antagonists in cardiac membranes.
...
...