Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex

@article{Lorenzen1993CharacterizationOM,
  title={Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex},
  author={Anna Lorenzen and Monika Nitsch‐Kirsch and Heidrun Vogt and Ulrich Schwabe},
  journal={Journal of Neurochemistry},
  year={1993},
  volume={60}
}
Abstract: A high‐affinity binding site for 5′‐N‐ethylcarboxamido[3H]adenosine ([3H]NECA) from bovine cerebral cortex has been characterized in its membrane‐bound and solubilized state after gel filtration on Sepharose CL‐6B. For detection of this site in membranes, it was necessary to remove metabolites with high affinities for this site enzymatically, e.g., adenosine by addition of adenosine deaminase and inosine by addition of nucleoside phosphorylase. The pore‐forming peptide antibiotic… 
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References

SHOWING 1-10 OF 36 REFERENCES
Characterization of the A2 adenosine receptor labeled by [3H]NECA in rat striatal membranes.
TLDR
The regional distribution of [3H]NECA binding and the affinities of adenosine agonists and antagonists for inhibition of binding indicate that the site labeled by [3NECA belongs to the high affinity, or A2a, subclass of A2 receptor.
Identification of the A2 adenosine receptor binding subunit by photoaffinity crosslinking.
TLDR
125I-labeled PAPA-APEC has identified the A2 receptor binding sub unit as a 45-kDa protein that is unique and distinct from the A1 binding subunit.
Ligand binding to the adenine analogue binding protein of the rabbit erythrocyte.
TLDR
Inhibition of [3H]adenosine binding by a series of 77 adenosine analogues was used to define the factors determining the binding affinity of this nucleoside.
Ligand binding to the adenine analogue binding protein of the rabbit erythrocyte.
TLDR
Inhibition of [3H]adenosine binding by a series of 77 adenosine analogues was used to define the factors determining the binding affinity of this nucleoside.
Interaction of Adenosine with Adenosine-Binding Protein, S-Adenosylhomocysteine Hydrolase
TLDR
This chapter is a brief review of the properties of AdoHcy hydrolase, with emphasis on the interaction of the enzyme with Ado.
Modification of receptor-mediated inhibition of adenylate cyclase in NG108-15 neuroblastoma X glioma cells by n-ethylmaleimide.
TLDR
Using membranes derived from NG108-15 neuroblastoma X glioma cells as a model system, the effect of NEM is examined on the interaction of three inhibitory receptors with adenylate cyclase and a 30-fold decrease in the potency of epinephrine is observed.
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