Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex

@article{Lorenzen1993CharacterizationOM,
  title={Characterization of Membrane‐Bound and Solubilized High‐Affinity Binding Sites for 5′‐N‐Ethylcarboxamido[3H]adenosine from Bovine Cerebral Cortex},
  author={A. Lorenzen and Monika Nitsch‐Kirsch and H. Vogt and U. Schwabe},
  journal={Journal of Neurochemistry},
  year={1993},
  volume={60}
}
Abstract: A high‐affinity binding site for 5′‐N‐ethylcarboxamido[3H]adenosine ([3H]NECA) from bovine cerebral cortex has been characterized in its membrane‐bound and solubilized state after gel filtration on Sepharose CL‐6B. For detection of this site in membranes, it was necessary to remove metabolites with high affinities for this site enzymatically, e.g., adenosine by addition of adenosine deaminase and inosine by addition of nucleoside phosphorylase. The pore‐forming peptide antibiotic… Expand
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