Characterization of Matrigel interfaces during defined human embryonic stem cell culture

@article{Kohen2009CharacterizationOM,
  title={Characterization of Matrigel interfaces during defined human embryonic stem cell culture},
  author={Naomi Kohen and Lauren Elizabeth Little and Kevin E Healy},
  journal={Biointerphases},
  year={2009},
  volume={4},
  pages={69-79}
}
Differences in attachment, proliferation, and differentiation were measured for human embryonic stem (hES) cells cultured on various substrata coated with Matrigeltm, a blend of extracellular matrix proteins derived from murine tumor cells. The authors observed that hES cells attach and grow poorly on Matrigel adsorbed onto polystyrene, while they proliferate when exposed to Matrigel adsorbed onto glass or oxygen plasma treated polystyrene (e.g., “tissue culture” treated polystyrene… CONTINUE READING

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