Characterization of Lysyl Residues of NADH-Cytochrome b5 Reductase Implicated in Charge-pairing with Active-site Carboxyl Residues

  • the Flavoprotein, Philipp StrittmatterS, Jason M. Kittler, Joanne E. Coghill
  • Published 2001


An expression vector for bovine NADH-cytochrome ba reductase was constructed from two DNA fragments that were derived from beef liver poly(A+) RNA using the polymerase chain reaction. Site-directed mutagenesis of the 3 lysine residues of the reductase, previously implicated in the formation of active-site charge pairs with carboxylate residues of cytochrome… (More)


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