Characterization of LC-HCC fusion protein of botulinum neurotoxin type A.

@article{Singh2011CharacterizationOL,
  title={Characterization of LC-HCC fusion protein of botulinum neurotoxin type A.},
  author={Manglesh Kumar Singh and Ram Kumar Dhaked and Padma Singh and Pallavi Gupta and Lokendra Singh},
  journal={Protein and peptide letters},
  year={2011},
  volume={18 3},
  pages={295-304}
}
Botulinum neurotoxins (BoNTs) are highly potent toxins that inhibit neurotransmitter release from peripheral cholinergic synapses. The gene for encoding the full length light chain with H(CC) (binding) domain of Clostridium botulinum neurotoxin A was synthesized and cloned into a bacterial expression vector pQE30-UA and produced as an N-terminally six-histidine-tagged fusion protein (rBoNT/A LC-H(CC)). This protein was expressed in two different strains of Escherichia coli namely BL21(DE3) and… CONTINUE READING