Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

@inproceedings{Wiedermannov2014CharacterizationOH,
  title={Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis},
  author={Jana Wiedermannov{\'a} and Petra Sudzinov{\'a} and Tom{\'a}{\vs} Kovaľ and Al{\vz}beta Rabatinov{\'a} and Hana {\vS}anderov{\'a} and Olga Ramaniuk and {\vS}imon Rittich and Jan Dohn{\'a}lek and Zhihui Fu and Petr Halada and Peter J Lewis and Libor Kr{\'a}sn{\'y}},
  booktitle={Nucleic acids research},
  year={2014}
}
Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD… CONTINUE READING