Characterization of GnRH receptors in bovine pituitary membranes.

  title={Characterization of GnRH receptors in bovine pituitary membranes.},
  author={Eli Hazum and D D Keinan},
  journal={Molecular and cellular endocrinology},
  volume={35 2-3},
Bovine pituitary gonadotropin-releasing hormone (GnRH) receptors were characterized and identified utilizing a superactive GnRH analog, Buserelin [( D-Ser(t-Bu)6, des-Gly10-ethylamide]-GnRH), and a photoreactive GnRH analog, [azidobenzoyl-D- Lys6 ]-GnRH. Both analogs bind with high affinity to a single class of receptors, with apparent IC50 values of 0.5 nM and 1 nM, respectively. The binding of 125I-labeled Buserelin to pituitary membranes was inhibited, in a dose-responsive manner, by both… CONTINUE READING