Characterization of Glutamate Decarboxylase (GAD) from Lactobacillus sakei A156 Isolated from Jeot-gal.

@article{Sa2015CharacterizationOG,
  title={Characterization of Glutamate Decarboxylase (GAD) from Lactobacillus sakei A156 Isolated from Jeot-gal.},
  author={Hyun Deok Sa and Ji Yeong Park and Seon-Ju Jeong and Kang Wook Lee and Jeong Hwan Kim},
  journal={Journal of microbiology and biotechnology},
  year={2015},
  volume={25 5},
  pages={696-703}
}
A gamma-aminobutyric acid (GABA)-producing microorganism was isolated from jeot-gal (anchovy), a Korean fermented seafood. The isolate, A156, produced GABA profusely when incubated in MRS broth with monosodium glutamate (3% (w/v)) at 37°C for 48 h. A156 was identified as Lactobacillus sakei by 16S rRNA gene sequencing. The GABA conversion yield was 86% as determined by GABase enzyme assay. The gadB gene encoding glutamate decarboxylase (GAD) was cloned by PCR. gadC encoding a glutamate/GABA… CONTINUE READING