Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes.

@article{Nassar1996CharacterizationOE,
  title={Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes.},
  author={Atef Nassar and Armelle Darrasse and M Lemattre and Alain Kotoujansky and Catherine Dervin and R{\'e}gine Vedel and Yves Bertheau},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 7},
  pages={2228-35}
}
Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and nonpectinolytic species and genera. The pel fragments amplified from the E. chrysanthemi strains studied were compared by performing a restriction fragment length polymorphism (RFLP… CONTINUE READING

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