Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli.

@article{Lara2017CharacterizationOE,
  title={Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli.},
  author={Alvaro R. Lara and Karim E. Ja{\'e}n and Juan-Carlos Sigala and Martina M{\"u}hlmann and Lars Regestein and Jochen B{\"u}chs},
  journal={ACS synthetic biology},
  year={2017},
  volume={6 2},
  pages={
          344-356
        }
}
Oxygen limitation can be used as a simple environmental inducer for the expression of target genes. However, there is scarce information on the characteristics of microaerobic promoters potentially useful for cell engineering and synthetic biology applications. Here, we characterized the Vitreoscilla hemoglobin promoter (Pvgb) and a set of microaerobic endogenous promoters in Escherichia coli. Oxygen-limited cultures at different maximum oxygen transfer rates were carried out. The FMN-binding… CONTINUE READING
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