Characterization of Drosophila heterochromatin. I. Staining and decondensation with Hoechst 33258 and quinacrine.

@article{Gatti1976CharacterizationOD,
  title={Characterization of Drosophila heterochromatin. I. Staining and decondensation with Hoechst 33258 and quinacrine.},
  author={Maurizio Gatti and Sergio Pimpinelli and Gino Santini},
  journal={Chromosoma},
  year={1976},
  volume={57 4},
  pages={351-75}
}
A number of preliminary experiments have shown that the fluorescence pattern of Hoechst 33258, as opposed to that of quinacrine, varies with the concentration of dye. The metaphase chromosomes of D. melanogaster, D. simulans, D. virilis, D. texana, D. hydei and D. ezoana have therefore been stained with two concentrations of H 33258 (0.05 and 0.5 mug/ml in phosphate buffer at pH 7) and with a single concentration of quinacrine (0.5% in absolute alcohol). The three fluorescence patterns so… CONTINUE READING

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