Characterization of DNA glycosylase activity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

@article{Darwanto2009CharacterizationOD,
  title={Characterization of DNA glycosylase activity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.},
  author={Agus Darwanto and Alvin Farrel and Daniel K. Rogstad and Lawrence C. Sowers},
  journal={Analytical biochemistry},
  year={2009},
  volume={394 1},
  pages={13-23}
}
The DNA of all organisms is persistently damaged by endogenous reactive molecules. Most of the single-base endogenous damage is repaired through the base excision repair (BER) pathway that is initiated by members of the DNA glycosylase family. Although the BER pathway is often considered to proceed through a common abasic site intermediate, emerging evidence indicates that there are likely distinct branches reflected by the multitude of chemically different 3' and 5' ends generated at the… CONTINUE READING