Characterization of DNA chips on the molecular scale before and after hybridization with an atomic force microscope

@inproceedings{Rouillat2005CharacterizationOD,
  title={Characterization of DNA chips on the molecular scale before and after hybridization with an atomic force microscope},
  author={Marie H{\'e}l{\`e}ne Rouillat and Vincent Dugas and Jean Rene Martin and Magali Phaner-Goutorbe},
  year={2005}
}
Using two different 25-mer oligonucleotide probes covalently grafted on a silicon substrate, we demonstrate how efficient atomic force microscopy (AFM) can be for monitoring each step of DNA chip preparation: from probe immobilization to hybridization on the molecular scale. We observed the probe-molecule organization on the chip after immobilization, and the target molecules, which hybridized with probes could be individually identified. This article presents a method of straightforwardly… CONTINUE READING
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