Characterization of Cyt2Bc toxin from Bacillus thuringiensis subsp. medellin.

@article{JurezPrez2002CharacterizationOC,
  title={Characterization of Cyt2Bc toxin from Bacillus thuringiensis subsp. medellin.},
  author={Victor Ju{\'a}rez-P{\'e}rez and Alejandra Guerchicoff and Clara Rubinstein and Armelle Del{\'e}cluse},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 3},
  pages={1228-31}
}
We cloned and sequenced a new cytolysin gene from Bacillus thuringiensis subsp. medellin. Three IS240-like insertion sequence elements and the previously cloned cyt1Ab and p21 genes were found in the vicinity of the cytolysin gene. The cytolysin gene encodes a protein 29.7 kDa in size that is 91.5% identical to Cyt2Ba from Bacillus thuringiensis subsp. israelensis and has been designated Cyt2Bc. Inclusions containing Cyt2Bc were purified from the crystal-negative strain SPL407 of B… CONTINUE READING