Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad.

@article{Kim2009CharacterizationOC,
  title={Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad.},
  author={Dal-Young Kim and H. Guzm{\'a}n and R. Bueno and J. Dennett and A. Auguste and C. Carrington and V. Popov and S. Weaver and D. Beasley and R. Tesh},
  journal={Virology},
  year={2009},
  volume={386 1},
  pages={
          154-9
        }
}
Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein… Expand
New Strains of Culex flavivirus Isolated in Argentina
TLDR
This study represents the first report of CxFV isolation and characterization in Argentina from the same geographic area where West Nile Virus has been detected andylogenetic analysis of nucleotide sequences showed that these strains are related closely to a CXFV strain isolated from Trinidad. Expand
Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States.
TLDR
Phylogenetic analysis showed that this new virus represents a distinct evolutionary lineage that belongs to the classical ISF group, and is provisionally named Culiseta flavivirus on the basis of its host association with Cs. Expand
Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil
TLDR
A novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil, is described andylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. Expand
Characterization of a novel flavivirus isolated from Culex (Melanoconion) ocossa mosquitoes from Iquitos, Peru.
We describe the isolation and characterization of a novel flavivirus, isolated from a pool of Culex (Melanoconion) ocossa Dyar and Knab mosquitoes collected in 2009 in an urban area of the AmazonExpand
First Identification of Culex flavivirus (Flaviviridae) in Brazil
TLDR
The circulation of CxFV in an urban setting where SLEV had previously caused an outbreak is reported, an important finding due to the assumption that the previous exposition of mosquitoes to CXFV might lessen the susceptibility of these mosquitoes to other flaviviruses. Expand
Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
TLDR
The discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. Expand
A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region
TLDR
Two strains of cell fusing agent virus (CFAV and CxFV) circulate in mosquitoes in Brazil, suggesting that a novel strain of CFAV is present in this region. Expand
Novel flaviviruses detected in different species of mosquitoes in Spain.
TLDR
A sensitive RT-nested polymerase chain reaction method is designed that amplifies a 1019bp fragment of the flavivirus NS5 gene and could be directly used in clinical or environmental samples for Flavivirus characterization and surveillance and contains enough phylogenetic information for proper taxonomic studies. Expand
Distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in Culex tarsalis Mosquitoes from western Canada with viruses isolated in California and Colorado.
TLDR
The ecology and geographic range of Calbertado virus warrants further study because it may potentially influence transmission of mosquito-borne flaviviruses, including important human pathogens such as West Nile and Saint Louis encephalitis viruses. Expand
Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean
TLDR
The detection of arboviral pathogens in this zone represents possible circulation and indicates a human health risk, demonstrating the importance of virological surveillance activities. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 25 REFERENCES
Culex Flavivirus Isolates from Mosquitoes in Guatemala
TLDR
Nucleotide sequence comparison of the eight flavivirus RNA-positive pools showed that there was 100% identity among them, and phylogenetic analysis of the NS5 and envelope gene regions indicated that they represent a strain of the recently described CxFV from Japan. Expand
Genetic characterization of a new insect flavivirus isolated from Culex pipiens mosquito in Japan.
TLDR
Phylogenetic analyses revealed that CxFV is closely related to the insect flaviviruses associated with Aedes mosquitoes, Cell fusing agent (CFA) and Kamiti River virus (KRV). Expand
Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico.
TLDR
Results from viral screening, and cell culture and molecular identification of the infected mosquitoes are presented, supporting previous suggestions of the existence of a large number of unsampled flaviviruses and elucidating the mechanism by which flaviviral sequences may have integrated into mosquito genomes. Expand
Isolation of a new flavivirus related to Cell fusing agent virus (CFAV) from field-collected flood-water Aedes mosquitoes sampled from a dambo in central Kenya
TLDR
This is the first isolation of a CFA-like virus from field-collected mosquitoes and indicates the presence of this group of viruses in nature. Expand
Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus
TLDR
The isolation of KRV demonstrates the presence of an insect-only flavivirus in nature and raises questions regarding potential interactions between this virus and other mosquito-borne viruses in competent vector populations. Expand
Phylogeny of the Genus Flavivirus
TLDR
The phylogenetic study revealed for the first time that from the putative ancestor two branches, non-vector and vector-borne virus clusters, evolved and from the latter cluster emerged tick-borne and mosquito-borne viruses clusters. Expand
Infection and Vertical Transmission of Kamiti River Virus in Laboratory Bred Aedes aegypti Mosquitoes
Abstract Kamiti river virus (KRV) is an insect-only Flavivirus that was isolated from field-collected Ae. macintoshi mosquitoes in 1999, and is closely related to cell fusing agent virus. Both ofExpand
Antigenic and genetic relationships among Rift Valley fever virus and other selected members of the genus Phlebovirus (Bunyaviridae).
TLDR
Comparison of serologic and genetic sequence analyses with five New World phleboviruses and Rift Valley fever virus demonstrated clear separation of these viruses from RVFV, into distinct phylogenies, based on sequences of the small, medium, and large RNA segments. Expand
Phylogeny of the genus flavivirus using complete coding sequences of arthropod-borne viruses and viruses with no known vector
TLDR
Comparative genetic amino acid alignments revealed that p-distance cut-off values of 0·330–0·470 distinguished the arthropod-borne viruses according to their recognized serogroups and Rio Bravo and Apoi virus were assigned to two distinct non-vectored virus groups. Expand
The complete nucleotide sequence of cell fusing agent (CFA): homology between the nonstructural proteins encoded by CFA and the nonstructural proteins encoded by arthropod-borne flaviviruses.
TLDR
It is suggested that CFA is an insect virus, which was present in the embryos from which the Ae. Expand
...
1
2
3
...