Characterization of Cellulose Production by a Gluconacetobacter xylinus Strain from Kombucha

@article{Nguyen2008CharacterizationOC,
  title={Characterization of Cellulose Production by a Gluconacetobacter xylinus Strain from Kombucha},
  author={V. Nguyen and B. Flanagan and M. Gidley and G. Dykes},
  journal={Current Microbiology},
  year={2008},
  volume={57},
  pages={449-453}
}
The aims of this work were to characterize and improve cellulose production by a Gluconoacetobacter xylinus strain isolated from Kombucha and determine the purity and some structural features of the cellulose from this strain. Cellulose yield in tea medium with both black tea and green tea and in Hestrin and Schramm (HS) medium under both static and agitated cultures was compared. In the tea medium, the highest cellulose yield was obtained with green tea (~0.20 g/L) rather than black tea (~0.14… Expand
Microbial Cellulose from a Komagataeibacter intermedius Strain Isolated from Commercial Wine Vinegar
In this study a new bacterial cellulose (BC) producer isolated from commercial vinegar is identified as Komagataeibacter intermedius JF2 based on the examination of general taxonomicalExpand
Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha
TLDR
Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type, which could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant. Expand
Bacterial Cellulose Synthesis in Kombucha by Gluconacetobacter sp and Saccharomyces sp
Strain Gluconacetobacter hansenii CGMCC1671 and Saccharomyces cerevisiae CGMCC1670 were applied to make traditional Kombucha with pure cultures to search for the optimum parameters of major factorsExpand
Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha
TLDR
The capacity of K. rhaeticus strain P 1463 to synthesise BC was found to be much higher than that of two reference strains for cellulose production, Komagataeibacter xylinus DSM 46604 and KomagATAeibacteria hansenii DSM 5602T, and was also considerably higher than the strain isolated from another Kombucha sample. Expand
Production of Bacterial Cellulose by Gluconacetobacterxylinus Using Taguchi Methods
The production of bacterial cellulose (BC) from Gluconacetobacter xylinus could be improved by the Taguchi method. Both the initial pH and glucose concentration are the important factors to affectExpand
Enhanced bacterial cellulose production from Gluconobacter xylinus using super optimal broth
The bacterial cellulose (BC) produced by Gluconobacter xylinus due to its versatile properties, is used in healthcare and industrial applications. However, its use is restricted owing to the limitedExpand
Production of Microbial Cellulose Films from Green Tea (Camellia Sinensis) Kombucha with Various Carbon Sources
The aim of this study was to evaluate the production of microbial cellulose films (MCFs) in culture media based on green tea and different carbon sources, using two microbial consortia (COr and CFr).Expand
Optimization of bacterial cellulose production by Gluconacetobacter xylinus using carob and haricot bean.
TLDR
It is revealed that BC production can be carried out using carob and haricot bean extracts as carbon and nitrogen sources, and CHb medium has higher buffering capacity compared to Hestrin and Schramm media. Expand
Production, Optimization and Partial Characterization of Bacterial Cellulose from Gluconacetobacter xylinus TJU-D2
PurposeThe aim of this work was to optimize and characteristic a bacterial cellulose (BC) produced by Gluconoacetobacter xylinus TJU-D2.MethodsAn one-factor test was conducted, then response surfaceExpand
Bacterial cellulose production by Acetobacter xylinum ATCC 23767 using tobacco waste extract as culture medium.
TLDR
Considering the huge disposal tobacco waste in China, the present study provides an alternative methodology to synthesize BC and showed the properties of BC obtained from TWE were similar to that from Hestrin-Schramm (HS) medium. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 20 REFERENCES
A Synthetic Medium for Bacterial Cellulose Production by Acetobacter xylinum subsp. sucrofermentans
Of several organic nitrogen sources tested, corn steep liquor (CSL) was found to be the most suitable for cellulose production by Acetobacter xylinum subsp. sucrofermentans BPR 2001. When lactate,Expand
Factors affecting the yield and properties of bacterial cellulose
TLDR
Physical properties of BC samples synthesized either in stationary cultures or in a horizontal fermentor revealed that cellulose from stationary cultures demonstrated a much higher value of Young's modulus, but a much lower value of water-holding capacity. Expand
Optimization of fermentation conditions for the production of bacterial cellulose by a newly isolated Acetobacter
TLDR
Addition of ethanol was found to eliminate the spontaneous mutation of Acetobacter sp. Expand
Production of Cellulose from D-Mannitol by Acetobacter xylinum KU-1
TLDR
It is found that Acetobacter xylinum KU-1 produced cellulose from D-arabitol and the productivity was more than 6 times as much as that of D-glucose. Expand
Effects of Acetan on Production of Bacterial Cellulose by Acetobacter xylinum
TLDR
It was concluded that acetan and BC syntheses were not directly related on the genetic level. Expand
Screening of Bacterial Cellulose-producing Acetobacter Strains Suitable for Agitated Culture
TLDR
No correlation between the amounts of cellulose accumulated in shaken and static cultures was observed, and the producer that yielded the highest cellulose accumulation in shaken culture was selected and named Acetobacter sp. Expand
SYNTHESIS OF CELLULOSE BY ACETOBACTER XYLINUM
TLDR
It is proposed that charged polysaccharides play a role similar to that of CMC in affecting the orientation of cellulose fibrils in the plant cell wall. Expand
SYNTHESIS OF CELLULOSE BY ACETOBACTER XYLINUM V. Ultrastructure of Polymer
TLDR
It is suggested that individual cellulose molecules are released at the cell surface and diffuse into the medium, wherein they finally enter into crystal-line patterns. Expand
NMR studies of acetan and the related bacterial polysaccharide, CR1/4, produced by a mutant strain of Acetobacter xylinum.
TLDR
2D NMR methods have been used to assign the 1H and 13C spectra of the two polysaccharides and to determine that CR1/4 has the structure shown below, which is identical to acetan. Expand
Cellulose-negative Mutants of Acetobacter xylinum
TLDR
Chemical mutagenesis induced a high frequency of cellulose-negative mutants of Acetobacter xylinum without significant cell killing, in agreement with the hypothesis that cellulose enables the cells to reach the surface of the liquid medium, where the supply of oxygen is abundant. Expand
...
1
2
...