Highly Influenced
5 Excerpts
Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential.
- Published 2001 in Biochemistry
Abstract
BphF is a small, soluble, Rieske-type ferredoxin involved in the microbial degradation of biphenyl. The rapid, anaerobic purification of a heterologously expressed, his-tagged BphF yielded 15 mg of highly homogeneous recombinant protein, rcBphF, per liter of cell culture. The reduction potential of rcBphF, determined using a highly oriented pyrolytic… (More)