Characterization of Atlantic halibut (Hippoglossus hippoglossus) Mx protein expression.

  title={Characterization of Atlantic halibut (Hippoglossus hippoglossus) Mx protein expression.},
  author={Veronica Bergan and B\orre Robertsen},
  journal={Developmental and comparative immunology},
  volume={28 10},
Mx proteins are antiviral GTPases that are induced by type I interferons in vertebrates. An Atlantic halibut Mx cDNA (HHMx) was recently cloned. In this work, a polyclonal antiserum against HHMx protein was generated that detected a 71 kDa protein in the nuclei of Chinook salmon embryo cells transfected with the HHMx cDNA. Mx protein expression in organs of halibut was studied by immunoblot analysis after injection with the double-stranded RNA poly I:C or infectious pancreatic necrosis virus… CONTINUE READING