Characterization of Arabidopsis thaliana cDNAs that render yeasts tolerant toward the thiol-oxidizing drug diamide.

@article{Kushnir1995CharacterizationOA,
  title={Characterization of Arabidopsis thaliana cDNAs that render yeasts tolerant toward the thiol-oxidizing drug diamide.},
  author={Sergei Kushnir and Elena Babiychuk and K Kampfenkel and E Belles-Boix and Marc Van Montagu and Dirk Inz{\'e}},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 23},
  pages={
          10580-4
        }
}
Diamide oxidizes cellular thiols and induces oxidative stress. To isolate plant genes which may, when overexpressed, increase tolerance of plants toward oxidative damage, an in vivo diamide tolerance screening in yeasts was used. An Arabidopsis cDNA library in a yeast expression vector was used to transform a yeast strain with intact antioxidant defense. Cells from approximately 10(5) primary transformants were selected for resistance to diamide. Three Arabidopsis cDNAs which confer diamide… CONTINUE READING

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