Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane.

@article{Sohlenkamp2002CharacterizationOA,
  title={Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane.},
  author={Christian Sohlenkamp and Craig C Wood and Gerhard W. Roeb and Michael K. Udvardi},
  journal={Plant physiology},
  year={2002},
  volume={130 4},
  pages={1788-96}
}
AtAMT2 is an ammonium transporter that is only distantly related to the five members of the AtAMT1 family of high-affinity ammonium transporters in Arabidopsis. The short-lived radioactive ion (13)NH(4)(+) was used to show that AtAMT2, expressed in yeast (Saccharomyces cerevisiae), is a high-affinity transporter with a K(m) for ammonium of about 20 microM. Changes in external pH between 5.0 and 7.5 had little effect on the K(m) for ammonium, indicating that NH(4)(+), not NH(3), is the substrate… CONTINUE READING
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