Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion.

@article{Rhie2003CharacterizationO2,
  title={Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion.},
  author={Alexandre G O Rhie and Louise Kirby and Natalie M Sayer and Rosanna Wellesley and Petra Disterer and Ian F. Sylvester and Andrew Gill and James Hope and William James and Abdessamad Tahiri-Alaoui},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 41},
  pages={39697-705}
}
We have isolated artificial ligands or aptamers for infectious prions in order to investigate conformational aspects of prion pathogenesis. The aptamers are 2'-fluoro-modified RNA produced by in vitro selection from a large, randomized library. One of these ligands (aptamer SAF-93) had more than 10-fold higher affinity for PrPSc than for recombinant PrPC and inhibited the accumulation of PrPres in near physiological cell-free conversion assay. To understand the molecular basis of these… CONTINUE READING